Micropropagation of Chrysanthemum (chrysanthemum Morifolium) Using Shoot Tip as Explant

Chrysanthemum has been cultivated for more than 2000 years and today, it is the world’s second most economically important floricultural crop following the rose (Teixeira da silva, 2003). Due to high popularity and demand for chrysanthemum it has become one of the first commercial targets for Micro propagation and thus tissue culture can be utilized for its large scale production. Regeneration of chrysanthemum plantlets was obtained by shoot tips as explant with different concentrations and combinations of auxins (IAA, IBA) and cytokinin (Kinetin), for the formation of micro-shoots, which were sub-cultured for development of roots. Fully developed plantlets were successfully transferred to suitable growing media for acclimatization. Among the various treatments for shoot regeneration, MS medium with Kinetin 3.0 mg l + IAA 2.0 mg l was found to be the best treatment combination as it produced 67.82 per cent success in 17.91 days. For root production, MS medium supplemented with IBA 1.0mg l was found to be the best treatment as it produced 82.12 per cent success in 19.19 days.